rRNA and Poly- -Hydroxybutyrate Dynamics in Bioreactors Subjected to Feast and Famine Cycles
نویسندگان
چکیده
rRNA and Poly-Hydroxybutyrate Dynamics in Bioreactors Subjected to Feast and Famine Cycles Dominic Frigon,† Gerard Muyzer, Mark van Loosdrecht, and Lutgarde Raskin* Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, and Environmental Biotechnology Group, Department of Biotechnology, Delft University of Technology, Delft, The Netherlands
منابع مشابه
rRNA and poly-beta-hydroxybutyrate dynamics in bioreactors subjected to feast and famine cycles.
Feast and famine cycles are common in activated sludge wastewater treatment systems, and they select for bacteria that accumulate storage compounds, such as poly-beta-hydroxybutyrate (PHB). Previous studies have shown that variations in influent substrate concentrations force bacteria to accumulate high levels of rRNA compared to the levels in bacteria grown in chemostats. Therefore, it can be ...
متن کاملPredicting the accumulation of storage compounds by Rhodococcus jostii RHA1 in the feast-famine growth cycles using genome-scale flux balance analysis
Feast-famine cycles in biological wastewater resource recovery systems select for bacterial species that accumulate intracellular storage compounds such as poly-β-hydroxybutyrate (PHB), glycogen, and triacylglycerols (TAG). These species survive better the famine phase and resume rapid substrate uptake at the beginning of the feast phase faster than microorganisms unable to accumulate storage. ...
متن کاملFast “Feast/Famine” Cycles for Studying Microbial Physiology Under Dynamic Conditions: A Case Study with Saccharomyces cerevisiae
Microorganisms are constantly exposed to rapidly changing conditions, under natural as well as industrial production scale environments, especially due to large-scale substrate mixing limitations. In this work, we present an experimental approach based on a dynamic feast/famine regime (400 s) that leads to repetitive cycles with moderate changes in substrate availability in an aerobic glucose c...
متن کاملSimulation of Bioreactors for PHB Production from Natural Gas
Recently, many economic studies of poly(3-hydroxybutyrate) PHB production on an industrial scale, and the impact of replacing petrochemical polymers by PHB were carried out, clearly indicating that the most crucial factors to reduce the cost of producing biopolymers are allotted to the application of microbial production strains capable of high productivi...
متن کاملFrequency dependent microbial growth during cycles of feast and famine
In Nature microbial populations are subject to fluctuating nutrient levels. Nutrient abundance fluctuations are important for evolutionary and ecological dynamics in microbial communities since they impact growth rates, population sizes and microbial physiology. Here we use automated continuous-culture devices and single-cell imaging to show that when populations of Escherichia coli are subject...
متن کامل